Course Outline
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- Significance: Reservoir, Pathogenesis, and Epidemiology
- Introduction: Background, Classification, and Safety
- Reservoir
- Pathogenesis
- Cutaneous and Subcutaneous Forms
- Pulmonary Forms
- Disseminated Forms
- Epidemiology
- The reservoir of actinomycetes is:
- Which aerobic actinomycetes are most commonly encountered among medical cases in the United States?
- Laboratories should use which biosafety level when processing aerobic actinomycetes?
- Laboratory Preparation of Specimens
- General Characteristics and Specimen Preparation
- All of the following are true of specimen preparation for isolation of the aerobic actinomycetes, except:
- Laboratory Identification: Microscopy
- Microscopy: Staining Techniques
- Microscopy: Staining Reactions
- Microscopy: Sulfur Granules
- A modified Kinyoun acid-fast staining technique uses:
- When viewing a direct Gram stain of suspected aerobic actinomycetes, all of the following may be seen, except:
- Laboratory Identification: Morphology and Testing
- Media for Propagating Aerobic Actinomycetes
- Primary Media
- Selective Media
- Primary isolation medium for sterile body site specimens include:
- After initial growth is achieved, subcultures of suspected aerobic actinomycetes should include which different incubation temperatures to determine t...
- Traditional Methods of Phenotypic Characterization
- Genotypic Identification Methods
- Chromatography
- Molecular Methods
- Polymerase Chain Reaction
- Other Molecular Tests
- In the identification of aerobic actinomycetes, HPLC methods are:
- Which of the following is a true statement?
- Serological Assays and MALDI-TOF
- Serological Assays
- MALDI-TOF
- Regarding the identification of aerobic actinomycetes, select the accurate statement:
- Regarding MALDI-TOF, select the incorrect statement:
- Description of Medically Important Aerobic Actinomycetes Genera and Species
- Susceptibility Testing and Treatment of Aerobic Actinomycetes Infections
- Susceptibility Testing: Methods and Limitations
- Susceptibility Testing: Typical Profiles
- Treatment
- Prevention
- Regarding susceptibility testing methods of the aerobic actinomycetes, which statement is false?
- For Nocardia, Streptomyces, and Actinomadura infections, the usual drug of choice is:
- References
Additional Information
Level of Instruction: Intermediate to advanced
Intended Audience: Medical laboratory scientists, medical
laboratory technicians, microbiologists, infection control members, and pathologists. This
course is also appropriate for MLS and MLT students and pathology residents.
Author Information: Dr. Julie Ann West is certified by the American Society for Clinical Pathology (ASCP) as a Medical Laboratory Scientist (MLS) and as a Specialist in Microbiology (SM). In addition, Dr. West has earned a PhD in Public Health - Infectious Disease Epidemiology - and is Certified in Public Health (CPH) by the National Board of Public Health Examiners. Dr. West is experienced as a Technical Specialist, Safety Officer, Educator, and Lead in the Veterans Administration.
The author has no
conflict of interest to disclose.
Reviewer Information:
Laurie Bjerklie, MA, MLS(ASCP)CM, is
a Lead Education Developer. She earned a B.S. in Medical Laboratory Science
from the University of North Dakota and an M.A. in Curriculum and Instruction
from Saint Xavier University. She has over 15 years of experience in higher
education and has held program director and faculty positions in both MLT and
MLS programs.
Nicole Darius, MLS(ASCP)CM, is experienced as a Microbiology Supervisor, Safety and Biosafety Officer, Antimicrobial Stewardship and Infection Control member, Lean certified, and Quality Assurance specialist in the Veterans Administration Healthcare System. She also has previous experience as a generalist medical laboratory scientist in chemistry, hematology, urinalysis, and blood bank.
Course Description: This course describes the significance of medically important aerobic actinomycetes. Microscopy, morphology, and identification methods are discussed. Susceptibility testing and treatment of infections are also included in this module. This module is intended for medical microbiologists with basic bacteriology experience.
