Home Products Most Popular Contact
No items in your cart.

Liver Biopsies: Anatomy and Histological Considerations (Online CE Course)

(based on 111 customer ratings)

Author: Brooke Eguia, BS, MS, HT(ASCP), HTL(ASCP)
Reviewer: Diana Harrington, BS, HT(ASCP)

The goal of this continuing education course is to provide essential knowledge to histology professionals regarding the structure and function of a liver biopsy received in the pathology laboratory. This course explores common diseases and provides information regarding how a liver biopsy assists the pathologist and clinician in diagnosing these diseases. Upon completion of this course, the histotechnologist will have a greater understanding of optimal histotechniques used to create slides that are adequate for diagnosis, develop a foundational knowledge about the anatomy of a liver biopsy (as well as common diseases), and also identify artifacts, special stains, and pigments that are specific to liver biopsies that may be used to assist in diagnosis.

See all available courses »

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1.5 hour(s)
Course number 578-035-16, approved through 4/30/2018
Course number 20-547763, approved through 9/1/2018

Objectives

  • Identify tissue and cellular components of a liver biopsy.
  • Describe characteristic histologic features observed in liver biopsies with common liver diseases.
  • Define common special stain applications performed on liver biopsies and list the diseases that the stains assist in identifying.
  • Explain critical factors that contribute to optimal technical preparation of liver biopsies.
  • Identify different pigments that can be seen in liver biopsies.

Customer Ratings

(based on 111 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • Introduction
  • Tissue Identification
  • Normal Histology
      • Lobular Organization: Portal Tracts and Central Veins
      • Portal Tracts: Bile Ducts
      • Portal Tracts: Arterioles
      • Portal Tracts: Portal Vein
      • Parenchyma: The "Lobule"
      • Parenchyma: Sinusoids
      • The portal vein provides a large percentage of the blood supply to the liver; the remaining blood is supplied to the liver via the hepatic artery.
      • The epithelial cells of the liver are grouped together to form functional units referred to as liver lobules.
  • Technical Preparation
      • Fixative
      • Rapid Tissue Processing: Transplant Patients
      • Biopsy Cycle Tissue Processing
      • Cassetting: Sponge versus Wrapping
      • Liver biopsies are delicate and subject to grossing artifacts. Using sponges to secure biopsy tissue in a tissue cassette has been proven to cause com...
  • Microtomy
      • Artifacts
      • Section Thickness
      • Step Sections
      • To aid in diagnosis, it is recommended that liver biopsy samples are sectioned at what micrometer (μm) thickness?
  • Disease
  • Stains
      • Trichrome
      • Reticulin
      • Rhodanine
      • Prussian Blue
      • Immunohistochemistry (IHC) and Immunoperoxidase (IP)
      • Masson's trichrome stain is used to demonstrate connective tissue in liver biopsies, staining collagen blue.
      • Reticular connective tissue cannot be visualized using the hematoxylin and eosin (H&E) stain. Which of the following is a silver stain used to ide...
  • Pigments
      • Exogenous Pigments
      • Endogenous Pigments
      • Which of the following is a yellow-brown, exogenous pigment found in increased amounts as cells age in organs such as nerves, heart, and liver?
      • Which of the following pigments are classified as endogenous? (Choose all that apply.)
  • References
      • References

Additional Information

Level of instruction: Basic to intermediate
 
Intended audience:  Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.  
 
Author information: Brooke Eguia, BS, MS, HT(ASCP), HTL(ASCP) is the Pathology Technical Supervisor at Fairview Southdale Hospital in Edina, Minnesota. She graduated from St. Cloud State University with a Bachelor of Science degree in general biology and from Capella University with a Master of Science in Human Service with a specialization in Health Care Administration. During her time as an undergraduate, Brooke's interests and studies focused on histologic techniques and gross anatomic dissection. Her senior year, she co-authored for aquatic toxicology research that Aquaculture published in January 2009. Acting as a primary health career mentor to high school students, Brooke satisfied her desire for training and teaching histotechnicians and also worked as adjunct faculty at Rasmussen College, as a Medical Assistant laboratory techniques instructor. Most recently, she has focused on proctoring histology students in clinical/classroom progress and exam preparation.
 
Reviewer information: Diana Harrington, BS, HT(ASCP) is a histotechnologist at The Dermatology Center of Indiana. Since graduating from Indiana University, she has worked in various hospital laboratories as a medical technologist and histotechnologist. Her experience led her to teach IU students as a Clinical Education Supervisor and create the Histotechnology Program as Program Director for Keiser University in Florida.
 
Course description: The goal of this continuing education course is to provide essential knowledge to histology professionals regarding the structure and function of a liver biopsy received in the pathology laboratory. This course explores common diseases and provides information regarding how a liver biopsy assists the pathologist and clinician in diagnosing these diseases. Upon completion of this course, the histotechnologist will have a greater understanding of optimal histotechniques used to create slides that are adequate for diagnosis, develop a foundational knowledge about the anatomy of

A liver biopsy (trichrome stain) demonstrating an increase in type I collagen in a patient with bridging portal fibrosis.
A liver biopsy stained with Gordon & Sweets reticulin stain demonstrating abnormal, reticulin collapse as well as abnormal arrangement of hepatocytes.
A liver biopsy stained with Gordon & Sweets reticulin stain demonstrating normal reticulin architecture, 40X magnification.
A liver biopsy stained with Prussian blue demonstrating ferric iron (+3) in a patient with hemosiderosis.
A trichrome stained liver biopsy demonstrating normal tissue architecture and favorable tissue cassetting.
DSCN9984
H&E stained liver biopsy demonstrating hepatocellular carcinoma (HCC) architecture.
SD12-489 cirrhosis with copper (3)

Keywords

These are the most common topics and keywords covered in Liver Biopsies: Anatomy and Histological Considerations:

ferric gomori necrosis histologically artery malignant cassettes ischemia consumption transfusion sinusoids hemosiderin iron adenoma nerve cellular sweets cassetting anchored ratio bile reticulin diagnose histotechnologist cells immunosuppressed non-alcoholic hematogenous grouped septate acute protein hepatocellular endoplasmic biopsy organisms non-blood vessels parenchyma stellate non-fatlike sinusoidal clinician gridley tumors hepatitis livers argyrophilic histologic wraps snook metabolism fetal melanin eosin hemotoxylin autoimmune hyalin sponges infection collagenous tracts gordon hyphae hemosiderosis cardiac conditions drug lipofuscin budding diagnostic discontinued non-viral pigments thickened fibrosis tumor nerves steatohepatitis trichrome lymphatic distinguishing wilder lobules vein pigment fatlike plentiful mallory immunohistochemistry liver reticulum biopsies grocotts infections needle cassette dietary candida acid-schiff methods red blood cells lobule pathologists pigmentation heart beta-pleated amyloid aspergillus body veins kupffer hepatocytes ascp infectious endogenous grossing mitochondria endothelial blood granulomatous carson exogenous serologic silver hampe knob rhodanine inflammatory disease hepatic honeycomb metabolic abnormal bilirubin organs massons benign artifact reagents diagnosis bone marrow rubeanic hemochromatosis alcohol clinical fungal epithelial cells histology granulomas carcinoma amyloidosis carbon nuclear histoplasma chemical formalin hematoxylin methenamine nonhematogenous laidlaw diseases cytoplasmic specimens reducer histoplasmosis hepatitis b cirrhosis artifacts wilsons
How to Subscribe
Histology CE Package$65 Add to cart
Individual course$20 Add to cart


A liver biopsy (trichrome stain) demonstrating an increase in type I collagen in a patient with bridging portal fibrosis.


A liver biopsy stained with Gordon & Sweets reticulin stain demonstrating abnormal, reticulin collapse as well as abnormal arrangement of hepatocytes.


A liver biopsy stained with Gordon & Sweets reticulin stain demonstrating normal reticulin architecture, 40X magnification.


A liver biopsy stained with Prussian blue demonstrating ferric iron (+3) in a patient with hemosiderosis.


A trichrome stained liver biopsy demonstrating normal tissue architecture and favorable tissue cassetting.


DSCN9984


H&E stained liver biopsy demonstrating hepatocellular carcinoma (HCC) architecture.


SD12-489 cirrhosis with copper (3)