The Human Leukocyte Antigen (HLA) System (Online CE Course)

(based on 317 customer ratings)

Ethan Kentzel, MS, MLS(ASCP)
Reviewers: Andrea Gordon, M.Ed., MT(ASCP)SH and Marissa Wildung, MLS(ASCP)CM

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This course covers the human leukocyte antigen system. The basic functionality of the immune system with emphasis on the role of the human leukocyte antigen (HLA) system is discussed along with the major histocompatibility complex which gives rise to HLAs. Principles and methodologies behind the various forms of HLA analysis are included. Also explored is the human leukocyte antigen's associative role in potential predisposition for disease, transplantation acceptance/rejection, graft-versus-host disease (GVHD), and alloreactivity.

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Approved through 9/30/2025
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Serology/Immunology): 1 hour(s)
Approved through 9/30/2025

Objectives

  • Describe the basic functionality of the immune system with emphasis on the role of the human leukocyte antigen (HLA) system.
  • Explain the major histocompatibility complex which gives rise to HLAs.
  • Compare and contrast the molecular structures of HLA molecules and describe their interactions with cells of the immune and other bodily systems.
  • Detail the principles and methodologies behind the various forms of HLA analysis.
  • Discuss the human leukocyte antigen's associative role in potential predisposition for disease, transplantation acceptance/rejection, graft-versus-host disease (GVHD), and alloreactivity.

Customer Ratings

(based on 317 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • Basics of the Immune System
  • Genomic Distribution of the HLA System
    • Chromosome Location
      • MHC/HLA Location on Human Chromosomes
      • Which of the following is not a locus that is found in HLA class I?
      • True or False: There are four regions on band 21 of chromosome 6 that give rise to HLA genes.
    • Inheritance Patterns
      • Inheritance Patterns of HLA Molecules
      • HLA Linkage Disequilibrium
      • Polymorphism
      • True or False: Crossover/recombination for HLA glycoproteins is very common, occurs during mitosis, and results in a different haplotype that the offs...
      • Match the following terminology with the appropriate definition.
  • Various Molecular Structures of the HLA System
      • Lymphocytes
      • Match the lymphocyte with its cluster of differentiation (CD).
    • Class I Structure and Functionality
      • HLA Class I: Structure and Location
      • HLA Class I: Functionality
    • Class II Structure and Functionality
      • HLA Class II: Structure and Location
      • HLA Class II: Functionality
      • Class I and II Structure and Function Summary
      • True or False: Class I HLAs are expressed on all nucleated cells and present endogenous antigens to lymphocytes. Class II HLAs are expressed on antige...
      • Match each of the functionalities described into the appropriate HLA class.
  • Methodologies of HLA Analysis
    • DNA Extraction and Quantitation
    • SSO
      • Sequence-Specific Oligonucleotide (SSO)
    • SSP
      • Sequence-Specific Primers (SSP)
    • Types of HLA Crossmatches
      • CDC Crossmatching (CDC XM)
      • Flow Cytometry Crossmatching (FXM)
    • Engraftment Monitoring
      • Engraftment Monitoring
      • Engraftment Monitoring, continued
    • Antibody Screening
    • NGS
      • Next Generation Sequencing
      • Match the testing with its methodology.
  • HLA's Role in Transplantation, Alloreaction, and Disease Associations
  • References
      • References

Additional Information

Level of Instruction: Beginner to Intermediate
Intended Audience: Medical laboratory scientists and technicians and other health care personnel who have an interest in this subject matter. This course is also appropriate for medical laboratory science students and pathology residents.
Author Information: Ethan Kentzel has earned a Master of Science in Biomedicine and Business at Drexel University.  He holds a BS degree in Medical Technology from Slippery Rock University and an ASCP MLS certification. Ethan has experience as a medical laboratory scientist working in various labs, including Blood Bank, Immunogenetics/HLA, Microbiology, and briefly as a generalist. While working as a laboratory scientist, he was also an adjunct professor for the Medical Laboratory Science program at St. Christopher's Hospital for Children. He works as a Program Director for the Medical Laboratory Science and Phlebotomy programs at Reading Hospital School of Health Science. He continues to teach at St. Christopher's Hospital for Children and Reading Area Community College.
Reviewer Information:
Andrea G. Gordon, M.Ed, MT(ASCP), SH, has over 25 years of experience as a Program Director for various laboratory science programs. She has approximately 5 years of experience as an administrator in higher education as a VP and Director of Education. Additionally, she has over 15 years of experience as a volunteer for NAACLS.
Marissa Wildung, MLS(ASCP)CM, is a Laboratory Supervisor with nine years of experience as a Medical Laboratory Scientist and a Faculty Instructor for an MLT/Phlebotomy Program in Central Minnesota. Marissa holds a Bachelor's in Medical Laboratory Science from the University of Cincinnati and is pursuing a Master's in Business Administration from St. Cloud State University.

How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 180 CE courses, most popular
$109Add to cart
Pick Your Courses
Up to 8 CE hours
$55Add to cart
Individual course$25Add to cart
Need multiple seats for your university or lab? Get a quote
Basic HLA inheritance


Chromosomal crossover during meiosis I (6).


DNA chromosomes of a human (4).


Class II function (11).