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- Basics of the Immune System
- History of the MHC in Humans and Other Animals
- Discovery of the Major Histocompatibility Complex (MHC) and Human Leukocyte Antigens (HLA)
- HLA 101 Terminology
- Immune System Overview
- HLA Nomenclature
- Nomenclature of HLA Glycoproteins
- Nomenclature of HLA Typings: Example
- The first field of an HLA nomenclature is designated as the allele group or the antigenic level of the protein that is detectable serologically.
- Genomic Distribution of the HLA System
- Chromosome Location
- Inheritance Patterns
- Inheritance Patterns of HLA Molecules
- HLA Linkage Disequilibrium
- Crossover/recombination for HLA glycoproteins is very common, occurs during mitosis, and results in a different haplotype that can be inherited to the...
- Match the following terminology with the appropriate definition.
- Various Molecular Structures of the HLA System
- Match the lymphocyte with its cluster of differentiation (CD).
- Class I Structure and Functionality
- HLA Class I: Structure & Location
- HLA Class I: Functionality
- Class II structure and Functionality
- Intro to HLA: mid-session review
- Class I HLAs are expressed on all nucleated cells and present endogenous antigens to lymphocytes, while Class II HLAs are expressed on antigen present...
- Which HLA glycoprotein is structurally characterized as a transmembrane protein with three heavy alpha chains and one light chain consisting of ß...
- Methodologies of HLA Analysis
- DNA Extraction and Quantitation
- Extraction of DNA
- Quantification of DNA
- Sequence Specific Primers (SSPs)
- Flow Cytometry Crossmatch and CDC Crossmatch
- Types of HLA Crossmatches: Flow Cytometry Crossmatching (FXM)
- Engraftment Monitoring
- Antibody Screening
- Serum Processing for HLA
- Antibody Screen and Percent Reactive Antibody Assay
- Antibody Screening Purpose and Interpretations
- Next Generation Sequencing
- Methodologies Questions
- Match the testing with its methodology.
- HLA's role in Transplantation, Alloreaction, and Disease Associations
- HLA Functionality and ABO/Rh in Transplantation
- Alloreactivity and Graft Versus Host Disease (GvHD)
- HLA Alleles and Predisposition to Diseases and Drug Reactions
- Match the types of rejection with the definition that meets the criteria for the type of reaction that would occur.
Level of instruction: Beginner to Intermediate
Intended Audience: Clinical laboratory technologists and technicians, and other health care personnel who have an interest in this subject matter. This course is also appropriate for clinical laboratory science students and pathology residents.
Author Information: Ethan Kentzel is completing his master's degree in Biomedicine and Business at Drexel University. He holds a BS degree in Medical Technology from Slippery Rock University, and holds an ASCP MLS certification. Ethan has over half a decade of lab experience as a medical technologist working in various labs including Blood Bank, immunogenetics/HLA, Microbiology, and briefly as a generalist. While working as a laboratory scientist, he was also an adjunct professor for the Medical Lab Science program St. Christopher's Hospital for Children. Currently, he works as an educator and Program Director for the Medical Laboratory Science and Phlebotomy programs at Reading Hospital School of Health Science. He continues to teach at St. Christopher's Hospital for Children and at Reading Area Community College.
Andrea G. Gordon, M.Ed, MT(ASCP), SH has over 25 years of experience as a Program Director for various laboratory science programs. She has approximately 5 years experience as an administrator in higher education as a VP and Director of Education. Additionally, she has over 15 years of experience as a volunteer for NAACLS.
Kevin F. Foley, PhD, DABCC, MT, SC is the director of clinical pathology for the Kaiser Permanente Northwest region. He also teaches clinical chemistry at Oregon Health Sciences University. Dr. Foley earned his PhD in clinical pharmacology and toxicology at East Carolina School of Medicine in North Carolina.