Histology Special Stains: Carbohydrates (Online CE Course)

(based on 84 customer ratings)

Author: Tanisha N. Neely, HT (ASCP)
Reviewer: Carla Shoffeitt, MSM, HT(ASCP)

This course is the first in a series of courses that will explore special stains used in the clinical histology laboratory. Basic carbohydrate staining mechanisms and classifying carbohydrates will be discussed. The material presented details the chemistry, diagnostic application and staining protocols for special stains used to demonstrate carbohydrates in tissue samples.

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number 578-019-16, approved through 3/31/2018
Course number 20-547697, approved through 9/1/2018


  • Explain basic staining mechanisms and the factors affecting dye binding.
  • Demonstrate a basic knowledge of carbohydrate classifications and the anatomic sites where certain types of carbohydrates may be detected.
  • Identify and troubleshoot special staining methods that are routinely used in the clinical histology laboratory to demonstrate carbohydrates in tissue samples.
  • Explain the clinical/diagnostic purpose for performing special staining procedures.

Customer Ratings

(based on 84 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • Introduction
  • Basic Staining Mechanisms
      • What Makes a Dye?
      • Factors That Affect Dye Binding
      • Acids, Bases, pH and Buffers
      • Basophilic and Acidophilic Staining
      • Oxidation and Reduction
      • Which of the following dye-binding factors is primarily responsible for reorganizing certain molecular structures in tissue samples so that they are m...
      • A staining solution with a pH of 5.5 would be considered _________.
  • Carbohydrate Classification and Tissue Detection
      • Carbohydrates
      • Classifying Carbohydrates
      • Monosaccharides
      • Polysaccharides
      • Mucopolysaccharides
      • Diagnostic Value of Demonstrating Mucopolysaccharides
      • ___________ is the primary polysaccharide demonstrated in animal/human tissue samples via histology staining techniques.
      • What are mucopolysaccharides most often referred to as?
  • Special Staining Methods Used to Demonstrate Carbohydrates in Tissue Samples
  • Using the Microwave for Special Staining Procedures
      • Microwaves in the Histopathology Laboratory
      • Tips for Using the Microwave for Special Staining Procedures
      • Safety Precautions for Microwave Usage
      • The container used to heat staining solutions in the microwave should be __________ .
  • References
      • References

Additional Information

Level of instruction: Intermediate

Intended Audience: Clinical laboratory histotechnologists and technicians,and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students,  pathology residents, and practicing pathologists.
Author information: Tanisha N. Neely, HT(ASCP) is a global histology technical liaison for Covance Central Laboratory Services. Prior to this position, she worked in hospital laboratories in histology and microbiology departments. Ms. Neely attended Indiana University where she earned an associates degree in General Studies and a Certificate in Histotechnology.  She is a member of the National Society for Histotechnology and the Indiana Society for Histotechnology.
Reviewer information: Carla J. Shoffeitt, MSM, HT(ASCP) is the Manager of the Pathology Department of Children’s Healthcare of Atlanta Pediatric Hospital in Atlanta, Georgia. She holds a Master of Science Degree in Healthcare Management as well as a Bachelor of Science degree in Biology from Troy University, Troy, Alabama. She is certified as a Histotechnician and has over 18 years of experience in the field of Histology.
Course Description: This course is the first in a series of courses that will explore special stains used in the clinical histology laboratory.

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