Click on the links below to preview selected pages from this course.
- Molecular Diagnostics
- Basis of Molecular Testing
- Writing the Code
- Types of Nucleic Acid Synthesis
- Human Genome
- Which of the following types of RNA is responsible for amino acid transport?
- Which of these nucleotide bases is present in DNA but is replaced by Uracil in RNA?
- Unique Terminology
- Terms and Definitions
- Match the following terms with the most appropriate response:
- The three base nucleotide sequence that provides the information necessary to identify an amino acid is termed a(n):
- Specimen Collection & Handling
- Specimen Collection and Handling
- Pre-analytical Variables
- Which of the following are examples of preanalytical variables that affect molecular methodologies?
- When collecting blood samples, one anticoagulant to avoid, especially when performing PCR is:
- Methodology Classifications
- Targets of interest can include:
- Classification Specific Procedures
- Procedure Principles
- Direct Nucleic Acid Testing Principle
- Direct Nucleic Acid Tests
- Amplified Nucleic Acid Testing Principle
- Amplified Nucleic Acid Tests
- Thermal Cycling
- Amplification Issues
- Factors Affecting Hybridization
- Match the following detection techniques with the most appropriate description:
- Which of the following steps is not included in a direct nucleic acid test?
- Match the following tests to their appropriate principle:
- Clinical Applications
- Why Choose a Molecular Method?
- When Nucleic Acids Get Altered
- Infectious Diseases
- Advantages of Molecular Testing
- Disadvantages of Molecular Testing
- What is the name of the substitution nucleic acid alteration that causes a coding for a different amino acid?
- Which of the following are considered advantages of molecular testing?
Level of instruction: Intermediate
Intended Audience: Clinical laboratory technologists and technicians, and other health care personnel who have an interest in this subject matter. This course is also appropriate for clinical laboratory science students and pathology residents.
Author Credentials: Lori Woeste, EdD
is an Assistant Professor in the Clinical Laboratory Science Program at Illinois State University
in Normal, Illinois
. She holds a Masters degree in Health and a Doctorate in curriculum and instruction. She has taught introductory and advanced courses in clinical chemistry and clinical biochemistry.
Reviewer Credentials: Beverly Barham is an Associate Professor in the Clinical Laboratory Science Program at Illinois State University
in Normal, Illinois
. Dr. Barham holds a Masters degree in Public Health and Doctorate in the combined areas of molecular biology, microbiology, and biochemistry. She has taught introductory and advanced courses in the Clinical Laboratory Science program including courses in immunology, immunohematology, clinical microbiology, parasitology, mycology, and advanced clinical microbiology.
Catherine Dragoni, MT(ASCP)SM received her BS degree in Medical Technology from the State University of New York, Upstate Medical Center, Syracuse. She began her career as a bench microbiologist at Maine Medical Center, Portland, Maine. Currently she is the Chief Technologist of Microbiology and Molecular Pathology at NorDx Laboratories, Scarborough, Maine.
Course Description: This course introduces you to the basic principles of molecular diagnostics and the associated terminology. Several direct and amplified nucleic acid test methods are presented and clinical applications are discussed.