Body Fluid Differential Tutorial (Online CE Course)

(based on 1,309 customer ratings)

Author: MaryBeth Helfrich, MT(ASCP)
Reviewer: Paul Fekete, MD, FCAP

If you have forgotten the art of differentiating choroid plexus cells from ependymal cells or viral lymphocytes from fluid monocytes, you are in luck! This course provides a thorough morphologic identification of over 40 cell types and disease states to recharge your memory. The course features over 100 crystal clear, perfectly stained body fluid images to illustrate both basic and advanced cellular morphology - perfect for a comprehensive, detailed review which can help refresh any technologist, both in or out of the hematology laboratory. The course is P.A.C.E. approved for 2 full credit hours to help reach your continuing education credit requirements. Enjoy learning and/or reviewing the key identification characteristics of body fluid cells in a user-friendly and fun format. Enroll today!

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 2 hour(s)
Course number 578-021-16, approved through 4/30/2018
Course number 20-295348, approved through 9/1/2016

Objectives

  • Compare and contrast the morphology of cells found in normal cerebrospinal fluid (CSF), normal pleural fluid, normal peritoneal fluid, and normal synovial fluid.
  • Identify the morphology of cells found in bronchoalveolar lavage (BAL).
  • Distinguish abnormal/reactive/infected cell morphology in fluids.
  • Recognize malignant cell morphology in body fluids.
  • Discuss appropriate scenarios for hematology/pathology review.

Customer Ratings

(based on 1,309 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • Cytocentrifugation Morphology
      • Cytocentrifugation of Body Fluid Samples
  • Cell Types Common To Most Types of Body Fluids
    • Neutrophils
      • Neutrophils
      • Neutrophils, continued
      • Eosinophils vs. Neutrophils
      • Nucleated blood cells will always have the same appearance on a cytocentrifuged smear as they do on a peripheral blood smear.
    • Lymphocytes, Atypical Lymphs, Plasma Cells
      • Normal Lymphocytes
      • Lymphocytes
      • Lymphocytes vs. Monocytes
      • Atypical Lymphocytes vs. Monocytes
      • Lymphocytes and Atypical Lymphocytes
      • Atypical Lymphocytes
      • Viral Lymphocytes
      • Lymphocytes and Plasma Cells in Body Fluids
      • The image on the right is a representative field from a cytospin preparation of a CSF from a patient with a viral infection. Identify the cell indicat...
    • Monocytes and Macrophages
  • Cells Found in Cerebrospinal Fluid
    • Cerebrospinal Fluid: Non-Blood Cells
    • Cerebrospinal Fluid: Bone Marrow Contamination
      • Bone Marrow Contamination in CSF
      • What is the identification of this cellular clump found in CSF? Note the presence of many similar-appearing nuclei without distinct lines of demarcati...
  • Cells Found in Pleural and Peritoneal Fluids
    • Mesothelial cells
  • Cells Found in Synovial Fluids
      • Synovial Lining Cells
      • Synovial Lining Cells, continued
  • Cells Found in Bronchoalveolar Lavages
    • Bronchial Lining Cells
      • Bronchial Lining Cells
      • Bronchial Lining Cells, continued
      • Match the following cell types to the body fluids in which they may be seen when the fluid is observed on a cytocentrifuged preparation.
  • Infectious Organisms in Body Fluids
    • Bacteria
      • Intracellular and Extracellular Bacteria
      • Intracellular Bacteria
      • Peritoneal Lavage Trauma
      • Trauma STAT
      • Septic Arthritis
    • Fungus
      • Yeast
      • Candida albicans - Germ Tube Formation
      • Hyphae
    • Toxoplasma
      • Central Nervous System (CNS) Toxoplasmosis
      • Central Nervous System (CNS) Toxoplasmosis, continued
      • The elements indicated by the arrows were observed on a Wright-stained smear of a synovial fluid. What is the likely identification of the elements an...
  • Malignant Fluids with Leukemia and Lymphoma.
  • Malignant Fluids with Metastatic Tumor
  • References

Additional Information

Level of Instruction: Intermediate
 
Intended Audience: This course is intended for laboratory professionals who have experience with peripheral blood morphology and basic experience with body fluid differential analysis.  This tutorial will provide a review of normal and abnormal body fluid morphology utilizing Wright-Giemsa stained cytospin preparations from cerebrospinal fluid (CSF), pleural, peritoneal and synovial fluids as well as bronchial alveolar lavage (BAL) samples.
 
Author Information: Marybeth Helfrich, MT(ASCP) is currently a Laboratory Technologist Specialist for the Hematology/Oncology Laboratory at Childrens Hospital of Philadelphia. She received her BS, MT from Temple University in Philadelphia and has nearly 40 years of experience in both adult and pediatric hematology and oncology settings. She is a regular presenter for ASCLS-PA, has been a presenter for ASCP (local and national), and the Texas Teleconference network. She is responsible for hematology morphology instruction and training for medical technologists and fellows. She is also a regular developer of morphology tutorials for in-house continuing education activities.

Reviewer Information: Paul Fekete, MD, FCAP is the President and CEO of MediaLab, Inc. He received his MD from Ohio State University and completed his clinical pathology residency at Emory University in Atlanta. He has served as an Assistant Professor of Pathology at Emory and as the Director of Laboratories for Gwinnett Medical Center in Lawrenceville, GA. He is currently President of Gwinnett Pathology Associates PC, Gwinnett Medical Center. Dr. Fekete is a member of Alpha Omega Alpha Honor Medical Society and a Fellow of the College of American Pathologists.

Keywords

These are the most common topics and keywords covered in Body Fluid Differential Tutorial:

intracellular nuclearcytoplasmic rectangular cerebrospinal fluid malignant cavities cilia leukemic lymphocyte macrophages samples bacteria morphology chromatin bacterial organisms plasmacytoid hospitals translocation respiratory cellular serous granules bi-nucleate nuclear bone marrow toxoplasmosis basophilic abnormal artifact burkitts peripheral blood smear peripheral blood smears ventricles synovial cavity nervous ependymal nucleus lymphocytes lymphoma effusions lymphoblastic microbiology histocytes pathology hematology irregularity spinal wbcs fluids histiocytes reactive lavage mesothelial cell vacuoles neuroblastoma granulation generous adenocarcinoma bronchial multinucleated primitive depicts hemorrhage choroid saline cytospins chemotherapy multinucleate diplococci disease cerebrospinal pneumonia infectious myeloid clusters monocyte body numbers cytospin technique cytospin hyphae cells circulating albicans membranes cytoplasmic rbcs shaggy white blood cells macrophage peritoneal differential leukemia fungal histiocyte sterile metastatic proportions tumor pericardial rhabdomyosarcoma nucleoli laboratory laboratorians nerve pleural mesothelial mesothelial cells lavages acute iron protocols demarcation diagnosis brain wright cytospin preparation mononuclear cells effusion clinical neuroectodermal differentials melanoma lymphoblasts clumps red blood cells mesothelium bals infection tumors vacuolation candida softer photos alveolar vacuolated arthritis blood neural textures infections lymphoid fused immature non-granular bowel hemosiderin burkitt monocytes nuclei cytoplasm identification cytospin preparations lymph trauma meningitis hospital neutrophils mycobacteria
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