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Body Fluid Differential Tutorial (Online CE Course)

(based on 1,448 customer ratings)

Author: MaryBeth Helfrich, MT(ASCP)
Reviewer: Paul Fekete, MD, FCAP

If you have forgotten the art of differentiating choroid plexus cells from ependymal cells or viral lymphocytes from fluid monocytes, you are in luck! This course provides a thorough morphologic identification of over 40 cell types and disease states to recharge your memory. The course features over 100 crystal clear, perfectly stained body fluid images to illustrate both basic and advanced cellular morphology - perfect for a comprehensive, detailed review which can help refresh any technologist, both in or out of the hematology laboratory. The course is P.A.C.E. approved for 2 full credit hours to help reach your continuing education credit requirements. Enjoy learning and/or reviewing the key identification characteristics of body fluid cells in a user-friendly and fun format. Enroll today!

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 2 hour(s)
Course number 578-021-16, approved through 4/30/2018
Course number 20-547597, approved through 9/1/2018

Objectives

  • Compare and contrast the morphology of cells found in normal cerebrospinal fluid (CSF), normal pleural fluid, normal peritoneal fluid, and normal synovial fluid.
  • Identify the morphology of cells found in bronchoalveolar lavage (BAL).
  • Distinguish abnormal/reactive/infected cell morphology in fluids.
  • Recognize malignant cell morphology in body fluids.
  • Discuss appropriate scenarios for hematology/pathology review.

Customer Ratings

(based on 1,448 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • Cytocentrifugation Morphology
  • Cell Types Common To Most Types of Body Fluids
    • Neutrophils
      • Neutrophils
      • Neutrophils, continued
      • Eosinophils vs. Neutrophils
      • Nucleated blood cells will always have the same appearance on a cytocentrifuged smear as they do on a peripheral blood smear.
    • Lymphocytes, Atypical Lymphs, Plasma Cells
      • Normal Lymphocytes
      • Lymphocytes
      • Lymphocytes vs. Monocytes
      • Atypical Lymphocytes vs. Monocytes
      • Lymphocytes and Atypical Lymphocytes
      • Atypical Lymphocytes
      • Viral Lymphocytes
      • Lymphocytes and Plasma Cells in Body Fluids
      • The image on the right is a representative field from a cytospin preparation of a CSF from a patient with a viral infection. Identify the cell indicat...
    • Monocytes and Macrophages
      • Monocytes
      • Monocytes vs Lymphocytes
      • Monocytes and Macrophages
      • Macrophages
      • Phagocytosis
      • Hemosiderin
      • Histiocytes
      • Macrophages are the phagocytes that originate from lymphocytes.
  • Cells Found in Cerebrospinal Fluid
    • Cerebrospinal Fluid: Non-Blood Cells
    • Cerebrospinal Fluid: Bone Marrow Contamination
      • Bone Marrow Contamination in CSF
      • What is the identification of this cellular clump found in CSF? Note the presence of many similar-appearing nuclei without distinct lines of demarcati...
  • Cells Found in Pleural and Peritoneal Fluids
  • Cells Found in Synovial Fluids
  • Cells Found in Bronchoalveolar Lavages
    • Bronchial Lining Cells
      • Bronchial Lining Cells
      • Bronchial Lining Cells, continued
      • Match the following cell types to the body fluids in which they may be seen when the fluid is observed on a cytocentrifuged preparation.
  • Infectious Organisms in Body Fluids
  • Malignant Fluids with Leukemia and Lymphoma.
  • Malignant Fluids with Metastatic Tumor
  • References
      • References

Additional Information

Level of Instruction: Intermediate
 
Intended Audience: This course is intended for laboratory professionals who have experience with peripheral blood morphology and basic experience with body fluid differential analysis.  This tutorial will provide a review of normal and abnormal body fluid morphology utilizing Wright-Giemsa stained cytospin preparations from cerebrospinal fluid (CSF), pleural, peritoneal and synovial fluids as well as bronchial alveolar lavage (BAL) samples.
 
Author Information: Marybeth Helfrich, MT(ASCP) is currently a Laboratory Technologist Specialist for the Hematology/Oncology Laboratory at Childrens Hospital of Philadelphia. She received her BS, MT from Temple University in Philadelphia and has nearly 40 years of experience in both adult and pediatric hematology and oncology settings. She is a regular presenter for ASCLS-PA, has been a presenter for ASCP (local and national), and the Texas Teleconference network. She is responsible for hematology morphology instruction and training for medical technologists and fellows. She is also a regular developer of morphology tutorials for in-house continuing education activities.

Reviewer Information: Paul Fekete, MD, FCAP is the President and CEO of MediaLab, Inc. He received his MD from Ohio State University and completed his clinical pathology residency at Emory University in Atlanta. He has served as an Assistant Professor of Pathology at Emory and as the Director of Laboratories for Gwinnett Medical Center in Lawrenceville, GA. He is currently President of Gwinnett Pathology Associates PC, Gwinnett Medical Center. Dr. Fekete is a member of Alpha Omega Alpha Honor Medical Society and a Fellow of the College of American Pathologists.

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csf 10 sml
csf atyp lymph vs mono sml2
corrected normal csf 1 sml2
lymhoblasts sml
synovial clump sml
trauma pert lavage sml2
burketts sml

Keywords

These are the most common topics and keywords covered in Body Fluid Differential Tutorial:

peripheral blood smears disease reactive clinical depicts nuclearcytoplasmic meningitis ventricles plasmacytoid lavages hyphae generous bowel diagnosis monocytes acute immature protocols lymphoma artifact malignant infection nuclei neural iron sterile granules identification nucleoli membranes circulating lymphocyte lymphoid pathology numbers macrophages clumps blood body neuroectodermal myeloid granulation mesothelial cells rhabdomyosarcoma leukemic histiocyte cytospin preparations monocyte histocytes fused trauma laboratorians shaggy cytospin mesothelial cell brain albicans non-granular melanoma multinucleated laboratory cerebrospinal fluid translocation alveolar mesothelium bacteria nervous cilia cavities toxoplasmosis peripheral blood smear wright tumor neuroblastoma bronchial cytoplasm morphology irregularity nucleus respiratory bacterial basophilic clusters multinucleate cerebrospinal leukemia cytospin technique bone marrow mycobacteria demarcation mononuclear cells burkitt pericardial peritoneal tumors microbiology hospitals saline wbcs lymphocytes chemotherapy samples bi-nucleate white blood cells spinal hemorrhage hematology cellular adenocarcinoma mesothelial abnormal effusion metastatic infections vacuoles lavage fluids textures cytospins cells pneumonia cytoplasmic chromatin rbcs cytospin preparation red blood cells lymph cavity organisms hospital vacuolated primitive proportions lymphoblastic neutrophils photos candida macrophage hemosiderin pleural diplococci fungal differentials nerve synovial nuclear choroid arthritis infectious burkitts intracellular bals ependymal vacuolation rectangular lymphoblasts effusions histiocytes serous softer differential
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anaplastic large cell 2 sml


csf 10 sml


csf atyp lymph vs mono sml2


corrected normal csf 1 sml2


lymhoblasts sml


synovial clump sml


trauma pert lavage sml2


burketts sml