Course Outline
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- Cytocentrifugation Morphology
- Cytocentrifugation of Body Fluid Samples
- Cell Types Common To Most Types of Body Fluids
- Neutrophils
- Neutrophils
- Neutrophils, continued
- Eosinophils vs. Neutrophils
- Nucleated blood cells will always have the same appearance on a cytocentrifuged smear as they do on a peripheral blood smear.
- Lymphocytes, Atypical Lymphs, Plasma Cells
- Normal Lymphocytes
- Lymphocytes
- Lymphocytes vs. Monocytes
- Atypical Lymphocytes vs. Monocytes
- Lymphocytes and Atypical Lymphocytes
- Atypical Lymphocytes
- Viral Lymphocytes
- Lymphocytes and Plasma Cells in Body Fluids
- The image on the right is a representative field from a cytospin preparation of a CSF from a patient with a viral infection. Identify the cell indicat...
- Monocytes and Macrophages
- Monocytes
- Monocytes vs Lymphocytes
- Monocytes and Macrophages
- Macrophages
- Phagocytosis
- Hemosiderin
- Histiocytes
- (True/False) Macrophages are the phagocytes that originate from lymphocytes.
- Match each cell type listed in the drop down box to the letter that corresponds to its identification in the image on the right.
- Cells Found in Cerebrospinal Fluid
- Cerebrospinal Fluid: Non-Blood Cells
- Cerebrospinal Fluid: Bone Marrow Contamination
- Bone Marrow Contamination in CSF
- What is the identification of this cellular clump found in CSF? Note the presence of many similar-appearing nuclei without distinct lines of demarcati...
- Cells Found in Pleural and Peritoneal Fluids
- Cells Found in Synovial Fluids
- Synovial Lining Cells
- Synovial Lining Cells, continued
- Cells Found in Bronchoalveolar Lavages
- Bronchial Lining Cells
- Bronchial Lining Cells
- Bronchial Lining Cells, continued
- Match the following cell types to the body fluids in which they may be seen when the fluid is observed on a cytocentrifuged preparation.
- Infectious Organisms in Body Fluids
- Bacteria
- Intracellular and Extracellular Bacteria
- Intracellular Bacteria
- Peritoneal Lavage Trauma
- Trauma STAT
- Septic Arthritis
- Fungus
- Yeast
- Candida albicans - Germ Tube Formation
- Hyphae
- Toxoplasma
- Central Nervous System (CNS) Toxoplasmosis
- Central Nervous System (CNS) Toxoplasmosis, continued
- The elements indicated by the arrows were observed on a Wright-stained smear of a synovial fluid. What is the likely identification of the elements an...
- Malignant Fluids with Leukemia and Lymphoma.
- Lymphoid Leukemia / Lymphomas
- Myeloid Leukemias
- Acute Myeloid Leukemia (AML)
- Acute Myeloid Leukemia (AML), continued
- Acute Monoblastic Leukemia (AMoL)
- Prominent vacuolation involving the cytoplasm of abnormal lymphoblast-like cells seen in a body fluid preparation is a distinctive feature of Burkitt ...
- Malignant Fluids with Metastatic Tumor
- References
Additional Information
Level of Instruction: Intermediate
Intended Audience: This course is intended for laboratory professionals who have experience with peripheral blood morphology and basic experience with body fluid differential analysis. This tutorial will provide a review of normal and abnormal body fluid morphology utilizing Wright-Giemsa stained cytospin preparations from cerebrospinal fluid (CSF), pleural, peritoneal and synovial fluids as well as bronchial alveolar lavage (BAL) samples.
Author Information: Marybeth Helfrich, MT(ASCP) is currently a Laboratory Technologist Specialist for the Hematology/Oncology Laboratory at Children's Hospital of Philadelphia. She received her BS, MT from Temple University in Philadelphia and has nearly 40 years of experience in both adult and pediatric hematology and oncology settings. She is a regular presenter for ASCLS-PA, has been a presenter for ASCP (local and national), and the Texas Teleconference network. She is responsible for hematology morphology instruction and training for medical technologists and fellows. She is also a regular developer of morphology tutorials for in-house continuing education activities.
Reviewer Information: Paul Fekete, MD, FCAP is the President and CEO of MediaLab, Inc. He received his MD from Ohio State University and completed his clinical pathology residency at Emory University in Atlanta. He has served as an Assistant Professor of Pathology at Emory, the Director of Laboratories for Gwinnett Medical Center in Lawrenceville, GA., and President of Gwinnett Pathology Associates. Dr. Fekete is a member of Alpha Omega Alpha Honor Medical Society and a Fellow of the College of American Pathologists.
