The page below is a sample from the LabCE course HER2 Testing in Breast Cancer. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Summary of HER2 Immunohistochemical (IHC) Method

The methodology described in this course is from the Dako HercepTest™, as used with the Dako Classic instrumentation with antigen recovery being performed outside of the instrument. Antigen recovery is necessary to release the aldehyde bonds caused by formalin fixation. Heat-induced epitope retrieval (HIER) is performed in a water bath at 100º C for 40 minutes using a citrate buffer. Polymer technology is used to reduce biotin related background and to increase the signal. The polymer is based on dextran technology and consists of both secondary goat anti-rabbit IgG and horseradish peroxidase molecules linked to a common dextran polymer.