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The page below is a sample from the LabCE course Hemoglobinopathies: Hemoglobin S Disorders. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Hemoglobin Electrophoresis Patterns in Sickle Cell Disorders (Acid)

The following list corresponds to this image of an acid hemoglobin electrophoresis.

Lanes 1 and 2 contain controls ASC and AF (Remember, AF and ASC are labels and do not indicate the order of migration.)

Lanes 3 and 4 (patient 1): Heterozygous sickle cell trait (Hb SA) Hb S is approximately 30%

Lanes 5 and 6 (patient 2): Double heterozygous Hb SC disease (Hb SC)

Lanes 7 and 8 (patient 3): Homozygous sickle cell disease (Hb SS)
Hb S is 100%

Lanes 9 and 10 (patient 4): Sickle cell hemoglobin with hereditary persistence of fetal hemoglobin. Hb S is approximately 70%; Hb F 30%.