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The page below is a sample from the LabCE course
Parasitology Case Studies: Protozoa in Blood (by ASCLS)
. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.
Learn more about Parasitology Case Studies: Protozoa in Blood (by ASCLS) (online CE course)
Laboratory Diagnosis
T. b. rhodesiense
trypomastigote stage can be found easily is blood
Numbers of parasites are usually numerous
Can be found in a biopsy of the chancre or in lymph nodes also
Giemsa stained smears or wet preps
See
http://youtu.be/EnsydwITLYk
to observe motile trypomastigotes
T. b. gambiense
is found usually by microscopic examination of a posterior cervical lymph node aspirate
Difficult to detect in blood
Concentration techniques and serial examinations are frequently needed
Examination of the buffy coat
Mini anion-exchange/centrifugation
Meningoencephalitic stage involves obsevation of trypanosomes in CSF
CSF WBC count of 5 per mm
3
or greated
Elevated CSF protein
Morphology
Centrally located nucleus
Undulating membrane
Anterior flagellum
Small posterior kinetoplast
Length of 14-33 µm
CDC/ Blaine Mathison
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