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The page below is a sample from the LabCE course ABO Typing Discrepancies. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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ABO Discrepancy: Example Two

Forward (Cell) Grouping

Reverse (Serum) Grouping

A1 Cells
B Cells

Group II - Discrepancies that are associated with unexpected or atypical reactions in the forward grouping, specifically mixed field reactions.

Most probable ABO type: Group A

Possible Causes: Weak subgroup of A, notably A3; weak results with mixed field reaction in the forward typing could be due to the transfusion of large quantities of O Red Blood Cells into a group A recipient, chimerism, or a group A patient transplanted with group O stem cells.

Some strategies for resolution: Obtain patient history including previous ABO typing results and transfusion history.