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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Picking Selected Panel Cells Conservatively

Choose cells that can help rule out more than one antibody at a time in order to help decrease supply usage and tech prep time.

Example: Ruling out C, Fyb, and M if you have a suspected Jka

Panel cell 90+0+0++0
Panel cell 100+++0+0+
Panel cell 11++0+0++0
Panel cell 12++++0++0

Instead of running 3 separate cells to rule out the antibodies, you can choose one that is homozygous positive for M, C, Fyb and negative for Jka. Panel cell 9 works in this case.

If the only antibody that is present is Jka, then your test results should be negative. If the results are positive then further rule outs will be needed to determine what is present.