Reactions are occurring strongly in IS phase but are weaker in AHG phase, which could be due to some disassociation of the cold antibody occurring at the warmer testing phase.
Prewarming of all reagents and sample will prevent binding of cold antibody. If the W+ reactions at AHG are due to residual cold antibody, the reactions should be negative with a prewarmed panel. No IS phase reading is performed.
Prewarmed Panel
