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The page below is a sample from the LabCE course Fundamentals of Molecular Diagnostics. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Thermal Cycling

Thermal cycling (thermocycling) uses specific instrumentation to cause variations in temperature during an amplification procedure in order to produce copies of the target of interest (or amplify the target of interest) so that detection can take place.

The temperature variations increase to unfold the DNA and then decrease to allow for reconnection or reannealing.