However limited or spacious the available area is, the establishment of a work process that directs the flow of both staff and specimens in a unidirectional flow is essential. Personnel should be educated to begin work in the "clean" area designated for reagent preparation; move on to the area designated for specimen preparation and extraction, and lastly, proceed to the area for amplification and detection.
Back and forth movement between these areas in the course of one run of specimens should NOT occur. Each area should have the necessary dedicated equipment to allow for the segregation of activities to their designated areas. Examples include pipette devices, small tabletop centrifuges, water baths, and racks. Gloves should be removed and disposed of before proceeding from one area to the next.