Sample type required: Deparaffinized and rehydrated tissue section (3-5 microns) on positively (+) charged slides
Preferred fixative: 10% neutral buffered formalin (NBF)
Working toluidine blue
|1 to 2 minutes||Solution: 0.1g toluidine blue + 100 mL distilled water|
|Distilled water||3 changes|
Post staining procedure: Tissue section should be rinsed well in distilled water and then dehydrated with 95% and absolute alcohols. Follow with two changes of xylene and then coverslip.
- Mast cells - Violet
- Other tissue elements - Shades of blue