The page below is a sample from the LabCE course Sexually Transmitted Bacterial Infections (by ASCLS). Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Sexually Transmitted Bacterial Infections (by ASCLS) (online CE course) »
How to Subscribe


  • Treponema pallidum ssp. pallidum are layered on a glass slide
  • Diluted patient serum is applied
  • Next anti-human globulin labeled with isothiocyanate is added
  • If antibodies are present in the serum, the spirochetes fluoresce green and can be seen in a fluorescent microscope