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The page below is a sample from the LabCE course Hemolytic Disease of the Fetus and Newborn. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Development of Anti-D

Development of anti-D may occur when Rh-negative individuals are exposed to D-positive red cells; however, development of anti-D varies greatly from person to person.

  • Some Rh-negative individuals produce anti-D after being exposed to a small volume of D-positive red cells (eg, 0.1 mL).
  • Some Rh-negative individuals must be exposed to a relatively large volume of D-positive cells to produce anti-D. 
  • Some Rh-negative individuals will never produce anti-D, regardless of exposure.