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Stains and Dyes


Stain or Dye



Ponceau S
Coomassie Brilliant Blue

Specific for Proteins

Silver is a biohazard


Sudan Black B
Oil Red O


Enzyme substrate and a
chromagen or fluorescent dye

Reaction catalyzed by enzyme and color or fluorescence detected


Not needed

Is intensely colored

Nucleic Acids (DNA/RNA)

  1. Ethidium Bromide (EtBr)
  2. SyBr Green
  3. Silver
  1. EtBr is Carcinogenic
  2. SyBr Green is new - Introduced in 1995
  3. Silver is a biohazard