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The page below is a sample from the LabCE course Immunohistochemistry (IHC) Basics in Histology. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Enzyme Blocking

Endogenous alkaline phosphatase activity is often found in lymphoid tissue, intestinal tissue and placenta. These endogenous enzymes can react with the substrate-chromogen used in the IHC staining procedure to produce a false-positive result. This is common with horseradish peroxidase (HRP) and alkaline phosphatase (AP) staining applications. To quench endogenous alkaline phosphatase activity, a 1mM concentration of levamisole can be added to the chromogen substrate solution.