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The page below is a sample from the LabCE course Histology Special Stains: Nervous Tissue. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Glial cells stained with Holzer's crystal violet. Image courtesy of Wikimedia Commons.

Holzer's Crystal Violet Staining - Chemistry

Holzer's crystal violet stain is used to identify glial cells and processes. Glial cells that have been stained with crystal violet are resistant to decolorization due to the alkalinity of the aniline-chloroform mixture.