Home Products Most Popular Contact
No items in your cart.
The page below is a sample from the LabCE course Cardiac Biomarkers. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Cardiac Biomarkers (online CE course) »
How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 123 CE courses, most popular
$95 Add to cart
Pick Your Courses
Up to 8 CE hours
$50 Add to cart
Individual course$20 Add to cart

Biomarker Sampling

Sample collection time and sample handling are important for cardiac markers. Blood samples should be collected and assayed in intervals. ESC/ACC guidelines stress the importance of serial sampling. They recommend sampling at presentation of chest pain (baseline), then at 6 - 9 hours, and if these samples are negative and necrosis is still suspected, repeat testing at 12 - 24 hours. Other studies recommend sampling at presentation, 2 - 4 hours, 6 - 8 hours, and at 12 hours.
Many laboratories will have serial troponin testing orderable as a panel (baseline, 30 min, 1 hour, 2 hour, etc.)
Troponin measurement should be available 24 hours a day and the results presented within 30 - 60 minutes after sample collection.
Point-of-care troponin testing is available that uses whole blood samples. Most large laboratories will also have larger instruments that perform plasma testing, these are typically more precise.
When using plasma, proper centrifugation is critical as small clots or debris are known to cause false-positive troponin results on some platforms. Once centrifuged, samples should be handled with care to avoid resuspension of any particulate matter that can interfere with the assay.