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The page below is a sample from the LabCE course Overview Of Major Antigens of the Rh Blood Group System. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Rh Testing For Blood Donors and Labeling Blood Products

Red blood cell donor units must be labeled for ABO and Rh. The donor's red cell D typing must be performed using anti-D reagent at the immediate spin phase. If the immediate spin test is negative, a test for weak D must be performed using 37o C incubation and an antihuman globulin (AHG) phase. If the immediate spin and weak D test are both negative, the donor is D negative and the unit is labeled Rh negative. If any part of the testing is positive, the donor is considered D positive and the unit is labeled Rh positive. The transfusing facility repeats immediate spin D typing on red cell products labeled as Rh(D) negative.
Harmening, DM,: Modern Blood Banking & Transfusion Practices, 6th ed. FA Davis, Philadelphia, PA, 2012, p 310.
Blaney, KD and Howard, PR: Basic & Applied Concepts of Blood Banking and Transfusion Practices, 3rd ed. Elsevier, St. Louis, MO, 2013, p 288.