Home Products Most Popular Contact
No items in your cart.
The page below is a sample from the LabCE course Hallmarks and Signaling of Cancer Cells. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Hallmarks and Signaling of Cancer Cells (online CE course) »
How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 111 CE courses, most popular
$95 Add to cart
Pick Your Courses
Up to 8 CE hours
$50 Add to cart
Individual course$20 Add to cart

Drug Screening: 2D Versus 3D?

The discovery of new drugs, including oncology drugs, starts with drug screening. Chemical screens are studies which are traditionally large-­scale endeavors carried out by big pharmaceutical companies such as Roche, Merck, Johnson & Johnson, Eli Lily, and GSK, just to name a few. However, in the past decade, academic screening centers have been established as well as many joint-ventures between pharmaceutical companies and academic institutions.
Traditionally, drug screening campaigns are conducted in two-dimensional (2-D) microtiter cell culture plates. In 2-D cell culture model systems, primary or immortalized cells are seeded in cell culture plates or dishes, which are chemically treated to help cells adhere and proliferate. While it is true that 2-D cell culture systems are easy to use and economical, the major disadvantage of 2D screening is that cells grow as monolayers, which is not at all how cells grow in vivo.
Recently, three-dimensional (3-D) cell culture systems have become an increasingly popular choice for drug screening. Despite the high cost and certain challenges in cell handling, 3-D systems closely mimic the cells in vivo:
  1. Cells in 3-D grow as multilayered spheres with enriched cell-cell contact. In contrast to 2-D culture (Fig. 7, left), Glioblastoma multiforme (GBM) cells grown in 3-D (Fig. 7, right) closely mimicing GBM tumor spheres in vivo.
  2. Cells in 3-D display very different response patterns to drug treatment. Cells tend to respond at higher levels of sensitivity to drug treatment in 2-D but tend to show reduced sensitivity in 3-D. Needless to say, this observation is of crucial clinical significance.