Bacillus anthracis: Other Diagnostic Tests to Aid in Preliminary Identification

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The page below is a sample from the LabCE course Preliminary Identification of the Primary Select Agents of Bioterrorism. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Bacillus anthracis: Other Diagnostic Tests to Aid in Preliminary Identification

Motility:
Motility by wet preparation is NOT recommended. Motility medium is more reliable, but shouldn't be read until 18-24 hours.
  • Bacillus anthracis is non-motile (NOTE: Bacillus cereus biovar anthracis has variable motility.)
  • Bacillus cereus is motile
Catalase:
Bacillus species are catalase positive. Catalase testing MUST be performed in a biosafety cabinet (BSC) due to the creation of aerosols.
India Ink:
India ink can reveal the capsule but testing is NOT recommended.
NOTE: Bacillus cereus biovar anthracis is similar and must also be ruled out.