Brucella species: Likely Clinical Specimens and Gram Stain Morphology - LabCE.com, Laboratory Continuing Education

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Brucella species: Likely Clinical Specimens and Gram Stain Morphology

Clinical specimens where organism may be encountered:

Blood
Bone marrow
Tissue

Gram stain morphology:

Very small, gram-negative coccobacilli
Stains very faintly and tends to retain crystal violet, especially in blood cultures
May initially be identified as gram-positive
Organism is larger than F. tularensis
Individual cells are evident

12. CDC/WA Clark. Image #1937. Caption: "This photomicrograph of a Gram-stained culture sample, revealed the presence of numerous Gram-negative, Brucella melitensis coccobacilli." PHIL 1977, public domain image. Accessed May 25, 2022.http://.phil.cdc.gov/Details.aspx?pid=1937

Gram stain of Brucella melitensis coccobacilli. (12)