Amplified Nucleic Acid Tests - LabCE.com, Laboratory Continuing Education

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Amplified Nucleic Acid Tests

Amplification Method	Amplifies	Use of Thermal Cycling (Thermocycling)
Polymerase Chain Reaction (PCR)	Target amplification using DNA polymerase	Yes
Ligase Chain Reaction (LCR)	Amplification of a short DNA probe using DNA ligase	Yes
Transcription- based or Transcription-mediated amplification(TMA)	Target amplification using reverse transcriptase and RNA polymerase	No
Strand Displacement (SDA)	Target amplification using DNA polymerase that continuously displaces strands of DNA containing the target sequence	No
Branched DNA (bDNA)	Signal amplification using alkaline phosphatase	No
Loop Mediated (LAMP)	Target amplification of multiple DNA sequences in a loop pattern using DNA polymerase	No
Nucleic acid sequence based (NASBA)	Target amplification using 3 enzymes	No
Q-beta Replicase	Probe amplification- The concentration of an RNA probe increases if the target is present	No