Medically Important Aerobic Actinomycetes (Online CE Course)

(based on 720 customer ratings)

Author: Julie Ann West, PhD (CPH [NEPHE]), MLS(ASCP)CM, SM(ASCP)CM
Reviewer: Nicole Darius, MLS(ASCP)CM

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This course describes the significance of medically important aerobic actinomycetes. Microscopy, morphology, and identification methods are discussed. Susceptibility testing and treatment of infections are also included in this module. This module is intended for medical microbiologists having basic bacteriology experience.

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Approved through 4/30/2025
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Microbiology/Mycology/Parasitology): 1 hour(s)
Approved through 4/30/2025

Objectives

  • Describe the significance of medically important actinomycetes (including reservoir, pathogenesis, and epidemiology), as well as proper safety protocol.
  • Microscopy: Demonstrate an understanding of the preparation of specimens and staining techniques with expected reactions for aerobic actinomycetes.
  • Morphology and Testing: Describe media, basic tests for phenotypic characterization, and several genotypic identification methods used to isolate and identify aerobic actinomycetes.
  • Describe the status of serological assays and MALDI-TOF.
  • Describe several genera and species of medically important aerobic actinomycetes. Recognize specific identifying characteristics of some notable aerobic actinomycetes.
  • Demonstrate an understanding of susceptibility testing and the treatment of human aerobic actinomycetes infections.

Customer Ratings

(based on 720 customer ratings)

Course Outline

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  • Significance: Reservoir, Pathogenesis, and Epidemiology of Medically Important Aerobic Actinomycetes
  • Laboratory Preparation of Specimens
  • Laboratory Identification: Microscopy - Staining Techniques and Reactions
      • Microscopy: Staining Techniques
      • Microscopy: Staining Reactions
      • Microscopy: Sulfur granules
      • A modified-Kinyoun acid-fast staining technique uses:
      • When viewing a direct Gram stain of suspected aerobic actinomycetes, all of the following may be seen, except:
  • Laboratory Identification - Morphology and Testing
    • Media for use in Propagating Aerobic Actinomycetes
      • Primary Media
      • Selective Media
      • Primary isolation medium for sterile body site specimens include:
      • After initial growth is achieved, subcultures of suspected aerobic actinomycetes to media should include which different incubation temperatures to de...
    • Traditional Methods of Phenotypic Characterization
      • Phenotypic Characterization
      • Media and Methods Used for Identification
      • More about Lysozyme...
      • More about Decomposition of Substrates...
      • All of the following tests may be used to aid in the phenotypic characterization of the aerobic actinomycetes, except:
      • Decomposition of substrates among the aerobic actinomycetes refers to:
    • Genotypic Identification Methods
      • Chromatography
      • Molecular Methods
      • PCR: NAATs and RFLP
      • Other Molecular Tests for Aerobic Actinomycetes
      • In the identification of aerobic actinomycetes, HPLC methods are:
      • Which of the following is a true statement?
  • Serological Assays and MALDI-TOF
      • Serological Assays
      • MALDI-TOF
      • Regarding the identification of aerobic actinomycetes - select the true statement from the list below:
      • Regarding MALDI-TOF - select the false statement from the statements listed below:
  • Description of Genera and Species of Medically Important Aerobic Actinomycetes
  • Susceptibility Testing and Treatment of Human Aerobic Actinomycetes Infections
      • Susceptibility Testing: Methods & Limitations
      • Susceptibility: Typical Profiles
      • Treatment
      • A Note about Prevention
      • Regarding susceptibility testing methods of the aerobic actinomycetes, which statement is false?
      • For Nocardia, Streptomyces, and Actinomadura infections, the usual drug(s) of choice is(are):
  • References
      • References

Additional Information

Level of Instruction: Intermediate/Advanced
Intended Audience: Medical technologists, and instructors experienced in bacteriology, graduate students in microbiology, clinical pathologists, and infectious disease physicians.
Course Description: This course describes the significance of medically important aerobic actinomycetes. Microscopy, morphology, and identification methods are discussed. Susceptibility testing and treatment of infections are also included in this module. This module is intended for medical microbiologists having basic bacteriology experience.
Author Information: Dr. Julie Ann West is certified by the American Society for Clinical Pathology (ASCP) as a Medical Laboratory Scientist (MLS) and as a Specialist in Microbiology (SM). In addition, Dr. West has earned a PhD in Public Health - Infectious Disease Epidemiology - and is Certified in Public Health (CPH) by the National Board of Public Health Examiners. Dr. West is experienced as a Technical Specialist, Safety Officer, Educator, and Lead in the Veterans Administration
Reviewer Information: Nicole Darius is certified by the American Society for Clinical Pathology (ASCP)CM as a Medical Laboratory Scientist. In addition, Nicole is experienced as a Microbiology Supervisor, Safety and Biosafety Officer, Antimicrobial stewardship and Infection Control member, Lean certified, and Quality Assurance specialist in the Veterans Administration Healthcare System. She also has previous experience as a generalist medical technologist in Chemistry, Hematology, Urinalysis, and Blood Bank.

How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 178 CE courses, most popular
$109Add to cart
Pick Your Courses
Up to 8 CE hours
$55Add to cart
Individual course$25Add to cart
Need multiple seats for your university or lab? Get a quote
4. Mycetoma of arm caused by Nocardia brasiliensis


14816 mycetoma of foot - Streptomyces infection


28. QUAD plate containing xanthine media; a halo appearance (hydrolysis of the media) is a positive result, while an absence of a halo is a negative result.


45. Streptomyces bacteria prep from a slide culture demonstrating long chains of small spores


48. Gram stain of Rhodococcus


54. Actinomadura pelletieri colony


5. Cellulitic inflammation of the foot becoming an actinomycotic mycetoma due to Nocardia.


43. Nocardia brasiliensis on agar