Urine is Not Sterile! Implications for the Clinical Microbiology Laboratory (by CACMLE) (Online CE Course)

(based on 39 customer ratings)

Paul Schreckenberger, PhD, D(ABMM), F(AAM)

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification) (CACMLE): 1.5 hour(s)
Course number 108-263-14, approved through 12/31/2017


  • Explain what is meant by the terms microbiota and microbiome.
  • List bacteria present in urine of asymptomatic women and women with overactive bladder that are urine "culture negative."
  • List bacteria present in urine of symptomatic patients with urinary tract infection that may be missed or misidentified using standard urine culture protocols.
  • Identify changes that may be needed in the way we perform urine cultures in the clnical microbiology laboratory.

Customer Ratings

(based on 39 customer ratings)

Course Outline

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  • PowerPoint Slides
      • Urine is Not Sterile! Implications for the Clinical Microbiology Laboratory
      • Urinary Tract Infection
      • Plating Urine on BAP
      • Colony Count Plate - BAP
      • Reporting Culture Results - by the Numbers?
      • Reporting Culture Results - by the Organism?
      • What is the Human Microbiota?
      • What is a Microbiome?
      • What is the Human Microbiome Project?
      • Is There a Urinary Microbiome?
      • Evidence of Uncultivated Bacteria in the Adult Female Bladder
      • Bladders of culture-negative women contain bacteria
      • Standard Urine Culture
      • Organism(s) Responsible for Urinary Symptoms
      • Standard Urine Culture
      • Expansion of Culture Techniques
      • Urine Collection Tubes for Anaerobes
      • Urine Collection Tubes for Anaerobes, cont.
      • JCM - Urine is Not Sterile - Enhanced Techniques
      • Overactive Bladder Syndrome
      • Overactive Bladder Syndrome, cont.
      • Control vs. OAB Baseline
      • Urine Culture BAP Plates
      • OAB Microbiota More Diverse Than Control
      • Number of Diverse Bacterial Species
      • Genera Frequency Chart
      • Significant Bacterial Species by Cohort
      • Case History #1
      • Case History #1 - Urine Culture
      • Aerococcus urinae - 48hr BAPs
      • Aerococcus urinae - Gram Stain
      • Aerococcus urinae - Gram Stain, cont.
      • Aerococcus ssp.
      • Aerococcus sanguinicola
      • Review Paper
      • Aerococci
      • Difficulties in Identification
      • Difficulties in Identification, cont.
      • Loyola Data
      • Loyola Data - Total Diagnosis, Yearly
      • Loyola Data - Specimen Types
      • Case History #2
      • Case History #2 - Past Medical History
      • Work-up
      • UA Results
      • Urine Culture - BAP
      • Urine Culture - 24 h BAP in CO2
      • Gram Stain
      • Microbiology Work-up
      • Culture Results: MALDI-TOF
      • Alloscardovia omnicolens
      • Site & Clinical Significance of Alloscardovia omnicolens
      • What's Next?
      • Loyola Urinary Research & Education Collaboration
      • The End
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