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The page below is a sample from the LabCE course PCR Fundamentals: Focus on Multiplex PCR Assay and the Advantages over Singleplex Assays. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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References

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Clinical and Laboratory Standards Institute (CLSI). Verification and Validation of Multiplex Nucleic Acid Assays; Approved Guideline. CLSI document MM-17A. Wayne, PA: CLSI; 2008.
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Elnifero EM, Ashshi AM, Cooper RJ, Klapper PE. Multiplex PCR: Optimization and Application in Diagnostic Virology. Clin Microbiol Rev. 2000;13:559-570.
Laboratory for Environmental Pathogens Research, Department of Environmental Sciences, University of Toledo. Polymerase Chain Reaction. 2004. Available at: http://www.eeescience.utoledo.edu/Faculty/Sigler/Von_Sigler/LEPR_Protocols_files/PCR.pdf. Accessed September 28, 2016.
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OIE Validation Guidelines 2014. Validation Guideline 3.6.3.: Development and Optimisation of Nucleic Acid Detection Assays. Available at: http://www.oie.int/fileadmin/Home/eng/Health_standards/aahm/current/GUIDELINE_3.6.3_NAD_ASSAYS.pdf. Accessed September 28, 2016.
Palavacino E. One Sample, Multiple Results: The Use of Multiplex PCR for Diagnosis of Infectious Syndromes. Clinical Laboratory News. Washington, DC: AACC; 2015. Available at: https://AACC.org/publications/cln/articles/2015/april/one-sample-multiple-results. Accessed September 28, 2016.
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