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The page below is a sample from the LabCE course Case Studies in Clinical Microbiology. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Additional Testing Information

A positive spot oxidase reaction is helpful in ruling out the oxidase-negative strains of Capnocytophaga ochracea and Acinetobacter baumannii, which in Gram stain may simulate Neisseria gonorrhoeae. Both Moraxella catarrhalis and Kingella kingae are oxidase positive; therefore, other tests must be used to differentiate these two species.
Neisseria "Look-Alike" Organisms Oxidase Result
A. baumaniiNegative
C. ochraceaNegative
K. kingaePositive
M. catarrhalisPositive

Superoxol is an additional spot test that may be helpful in the presumptive identification of N. gonorrhoeae.
Superoxol is 30% hydrogen peroxide, in contrast to the 3% solution that is used in the catalase test. Other Neisseria species and M. catarrhalis are either negative for this test or give a weak, delayed reaction.