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Listeria motility

Listeria monocytogenes is optimally motile at 25° C; and is non-motile at 35-37° C. Motility may be directly assessed by observing bacterial cells with a tumbling motion in a direct mount preparation. In soft motility agar, the identification can be made by observing for a thin, umbrella-like lateral extension of growth from the stab line (noted by arrows in the image).