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The page below is a sample from the LabCE course Case Studies in Clinical Microbiology. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Clostridium septicum RapID ANA

  • The definitive identification of C. septicum can be made using a profile of biochemical reactions, as is contained in the RapID ANA strip seen in the image. The upper set of tubules are reactions before addition of reagents; the bottom set of reactions after reagents are added.
  • The upper set of letter codes is used to read the reactions before addition of reagents; the lower set of labels indicate the tests to read following addition of reagents.
  • Of all the reactions included, only ONPG and NAG in the upper set are positive.
  • The biotype number derived from this profile of reactions, 014000 codes for Clostridium septicum, thus confirming the identification.