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Enterococcus faecium
Identification
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The page below is a sample from the LabCE course
Case Studies in Clinical Microbiology
. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.
Learn more about Case Studies in Clinical Microbiology (online CE course)
Enterococcus faecium
Identification
As a high percentage of
Enterococcus faecium
strains carry the Van A gene and are highly resistant to vancomycin, species identifications are performed in some laboratories where MIC susceptibility testing may not be available.
Methods for the phenotypic separation of
E. faecium
from
E. faecalis
are limited.
Illustrated in this image are positive reactions for acid production from arabinose and melibiose (yellow color), characteristic of
E. faecium
.
E. faecalis
are negative for these reactions.
A few preformed substrates, such as beta galactosidase (
E. faecium
positive,
E. faecalis
negative), also serve to separate these two species, accomplished by certain commercial systems that include these substrates.
E. faecium
is not motile, an additional characteristic helpful to separate vancomycin-resistant
Enterococcus
(VRE) species from
E. cassiloflavus
and
E. gallinarum
, both of which are motile, and carry the low level resistant gene VAN-c.
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