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ESBL Screening by Broth Microdilution
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The page below is a sample from the LabCE course
Case Studies in Clinical Microbiology
. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.
Learn more about Case Studies in Clinical Microbiology (online CE course)
ESBL Screening by Broth Microdilution
For screening of
K. pneumonia
,
K. oxytoca
, and
E. coli
isolates by broth microdilution, the use of cefpodoxime (4 µg/mL), ceftazidime (1 µg/mL), aztreonam (1 µg/mL), cefotaxime (1 µg/mL), or ceftriaxone (1 µg/mL) is proposed. For
P. mirabilis
isolates, the use of cefpodoxime (1 µg/mL), ceftazidime (1 µg/mL), or cefotaxime (1 µg/mL) is proposed. Again, for screening purposes in all of these organisms, the use of more than one of these agents improves the sensitivity of detection.
For
K. pneumonia
,
K. oxytoca
, and
E. coli,
MIC
growth ≥ 2 µg/mL ceftazidime, aztreonam, cefotaxime, or ceftriaxone or MIC ≥ 8 µg/mL for cefpodoxime may indicate ESBL production and phenotypic confirmation should be performed. For
P. mirabilis
, an MIC ≥ 2 µg/mL for cefpodoxime, ceftazidime, or cefotaxime may indicate ESBL production and phenotypic confirmation should be performed.
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