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The page below is a sample from the LabCE course Pituitary Hormones. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Laboratory Analysis of ACTH

Plasma ACTH measurements are used in the differential diagnosis of pituitary insufficiency and treatment of certain disorders of the adrenal glands, such as Cushing syndrome and adrenocortical insufficiency (Addison's disease).
Measurement of ACTH is most commonly performed using immunoassay methods that employ radioisotopic (RIA) or chemiluminescent (CIA) modes of detection. Both RIA and CIA analytical principles have demonstrated the ability to distinguish between low-normal and suppressed ACTH secretion from the pituitary.