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The page below is a sample from the LabCE course Pituitary Hormones. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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The Laboratory's Role in Assessing Pituitary Function

Physicians rely on accurate, sensitive, and specific measurement of the pituitary hormones to aid in the diagnosis of pituitary diseases and to monitor the effectiveness of treatments. Over the past 10-20 years, improved analytical specificity for measuring the hormone of interest, and the enhanced analytical sensitivity to detect very small concentrations of hormones has been the focus of manufacturers to provide reliable clinical laboratory results.
On the following pages, a brief overview of the historical and current technologies is provided.
Analytical PrinciplePituitary Hormone
Radioimmunassay (RIA)
ADH
ACTH
GH (historic)
FSH (historic)
LH (historic)
PRL
TSH (historic)
Enzyme Immunoassay (EIA)
ACTH
GH
FSH
LH
PRL
TSH (2nd generation)
Chemiluminescent Immunoassay (CIA)
ACTH
GH
FSH
LH
PRL
TSH (3rd generation)
Chromatographic
ADPH
Oxytocin
GH
FSH
LH
PRL