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Use of Image Analysis to Reduce Analytical Phase Testing Inaccuracies

Image analysis can be an effective tool for achieving consistent interpretation. The following are important considerations:
  • A pathologist must confirm the image analysis result.
  • Image analysis procedures must be validated before implementation.
  • Image analysis equipment, just as other laboratory equipment, must be calibrated and subjected to regular maintenance and internal quality control (QC) evaluation. A lack of calibration of the optical microscopes used by pathologists would certainly contribute to interpretive variation.
  • If pathologists use several different microscopes to read assays, a system of calibration of these instruments should be implemented to ensure consistent interpretation.