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The page below is a sample from the LabCE course Introduction to Flow Cytometry: Blood Cell Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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T-Helper Cell Example

Mature T cells can be divided into two major types:

  • Helper cells
  • Suppressor cells (cytotoxic T cells)

The orange labeled MoAbs are specific for the CD3 antigen on T cells, and the green labeled MoAbs are specific for the CD4 antigen on T cells. When a true T-helper cell is analyzed in the flow cytometer, it would emit both colors after it passes through the laser, as illustrated in the image on the right.