The page below is a sample from the LabCE course Histology Special Stains: Carbohydrates. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Histology Special Stains: Carbohydrates (online CE course) »
How to Subscribe
Histology CE Package$65 Add to cart
Individual course$20 Add to cart

PAS with diastase; liver

Periodic Acid-Schiff (PAS) with Diastase: Diagnostic Applications

The primary purpose of using the PAS with diastase staining procedure is to differentiate glycogen from other PAS-positive elements, such as mucin, that may be present in the tissue sample. Mucin can be specifically identified in certain tissue samples using the PAS staining procedure only if the glycogen (which is also PAS-positive) is digested with diastase and washed out.

Several enzyme deficiencies can be diagnosed through the analysis of glycogen deposits in the liver.

The PAS with diastase staining procedure can also be used to differentiate glycogen granules from other granules in various tumor types.