The page below is a sample from the LabCE course Autoimmune Diseases and Antinuclear Antibody Testing: Methods and Staining Patterns. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Common Cytoplasmic Staining Patterns (continued)

Anti-golgi pattern (AC-22)
Coarse granular staining around one side of nucleus is characteristic of an anti-Golgi antibodies pattern. This pattern is reported as "ANA negative, suspect anti-golgi antibodies present." No follow-up testing is necessary. Anti-golgi antibodies are seen in various diseases.
Anti-mitotic spindle pattern
Anti-mitotic spindles antibodies refers to several different patterns which, in a broad sense, includes CENP-F, Centromere and Midbody. However, in the case of the cytoplasmic patterns it only applies to the staining of the spindle fibers. This pattern may appear in various autoimmune disorders. It is reported as "Suspect mitotic spindle."
Anti-NuMA pattern (AC-26)
This pattern produces a speckled ANA along with staining of the spindle fibers. It is reported as "ANA positive, speckled, suspect mitotic spindle antibodies present." Follow-up testing to rule out possible antibodies to the ENAs is suggested. Titering is necessary.
Anti-HsEg5 pattern (AC-25)
Antibodies to HsEg5 are ANA negative and only stain the spindle fibers. This pattern is reported out as "ANA negative, suspect mitotic spindle antibodies present." No follow-up testing is necessary and titering is optional.