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Homogeneous (AC-1)

This is an example of a homogeneous ANA pattern.

This pattern is characterized by smooth staining in the nucleus of the interphase cells (a). The nucleoli may or may not stain. Notice the smooth staining in the chromosomal area of the metaphase mitotic cells (b).

Follow-up testing for anti- dsDNA, histone or chromatin antibodies is recommended. These antibodies are primarily seen in patients with systemic lupus erythematosus (SLE).

This pattern is reported as "ANA positive, Homogeneous; titering is necessary."

Homogeneous:

Interphase cells

  • Smooth staining of entire nucleus
  • Nucleoli may or may not stain

Metaphase mitotic cells

  • Smooth staining of the chromosomal region
  • No staining outside of the chromosomal area