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Antigen Chart

Pattern observed by indirect immuno fluorescence Antigen Disease(s) in which antibodies are seen Routine tests used to confirm specific antibody
Homogeneous Double stranded DNA (dsDNA) Characteristic of SLE, lower levels in other rheumatic diseases IFA or CZ using Crithidia luciliae, RIA, ELISA, addressable laser bead assay (ALBIA)
Nucleosome or chromatin SLE, drug-induced LE ELISA
Histone Drug-induced LE, SLE ELISA, ALBIA
Unusual homogeneous Nuclear membrane Lupoid hepatitis ELISA for gp-210
Speckled Sm (Smith) Marker antibody for SLE Immunodiffusion (ID), ELISA, ALBIA
U1-RNP High levels in MCTD and SLE, low levels in other rheumatic diseases ID, ELISA, ALBIA
Speckled (and/or SSA pattern if using HEp-2000®)-Can also be ANA negative SS-A/Ro High prevalence in Sjögren syndrome sicca complex, lower prevalence in other rheumatic diseases With HEp-2000 characteristic ANA pattern is confirmatory, others confirm with ID, ELISA, ALBIA
Fine speckled or ANA negative Ro52 Sjögren syndrome, myositis, Neonatal Lupus ELISA, ALBIA
Fine speckled (sometimes with nucleolar staining as well) SS-B/La High prevalence in Sjögren syndrome sicca complex, lower prevalence in other rheumatic diseases ID, ELISA, ALBIA
Fine speckled, homogeneous, nucleolar Scl-70 Marker antibody for Scleroderma ID, ELISA, ALBIA
Cell cycle dependent speckled PCNA Marker antibody for SLE ID, ELISA, ALBIA
Coarse speckled Nuclear matrix Seen in some patients with evolving connective tissue disease NONE
3-20 dots NSp I, sp-100, MND, PBC 95 Associated with primary biliary cirrhosis ELISA, ALBIA
Cell cycle dependent speckled with speckling in metaphase mitotics NSp II, CENP F Some association with malignancies NONE
Staining in cleavage furrow between dividing cells Midbody Unknown Confirm by staining pattern
Centromere CENP A, CENP B, CENP C Seen in 57-82% of patients with limited form (CREST) of scleroderma and Raynaud phenomenon Confirmed by staining pattern ELISA, ALBIA
Nucleolar Fibrillarin (clumpy nucleolar) Scleroderma ELISA, ALBIA
RNA polymerase I, NOR-90, others? (speckled nucleolar) Scleroderma and other connective tissue diseases ELISA, ALBIA
PM-1 (PM/Scl), others?(smooth nucleolar) Polymyositis/Scleroderma overlap ELISA, ALBIA