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The page below is a sample from the LabCE course Breast Cancer Predictive Markers. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Variables Affecting Immunohistochemistry (IHC)

All factors related to each phase of specimen processing may affect IHC staining.
Critical variables which affect staining results include:
  • Temperature
  • pH
  • Ionic conditions
  • Titer of concentration of the antibody
  • Number of epitope sites per cell
  • Duration of incubation
  • Fixation and Tissue Processing