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The Matrix Interferents

Within the sample matrix itself exists components that occur pre-analytically that may affect the accurate analysis of the intended measurand.

Matrix Effect

Interfering Substance

Hemolysis (A)

Hemoglobin

Lipemia (B)

Triglycerides and chylomicrons

Icterus (C)

Bilirubin

Viscosity

Protein and immunoglobulins


For many analytes, interferent studies have been performed by the manufacturer of the assay or analytical reagents to test the effects of these matrix interferents at various concentrations of the interfering substances. For laboratory-developed tests, or tests for which such interference studies are not available, the laboratory should perform interference testing in accordance with the CLSI recommendations.