Forward (Cell) Grouping | Reverse (Serum) Grouping |
4+ | 0 | 4+ | 2+ | 4+ |
Unexpected reactions (including rouleaux) in reverse grouping.
Most probable ABO type: Group A
Possible causes: A2 patient with an anti-A1, abnormal serum proteins resulting in rouleaux formation, a room temperature reactive alloantibody, or a cold autoantibody.
Some strategies for resolution: Perform an antibody screen; test patient cells with anti-A1 lectin; or perform saline replacement techniques.