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The page below is a sample from the LabCE course ABO Typing Discrepancies. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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ABO Discrepancy: Example One

Forward (Cell) Grouping

Reverse (Serum) Grouping

A1 Cells
B Cells

Category: Group l-- Discrepancies that are associated with unexpected or missing/atypically weakened reactions in the reverse typing.

Most probable ABO type: Group A

Possible causes: Immunocompromised, age-related, transplantation, dilution, technical/procedural error

Some strategies for resolution: Repeat testing with careful attention to accuracy or repeat reverse typing with extended incubation at or below room temperature. Weak ABO reactions can often be potentiated using extended incubation at or below room temperature.