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ABO Discrepancy: Example One

Forward (Cell) Grouping

Reverse (Serum) Grouping

Anti-A
Anti-B
Anti-A,B
A1 Cells
B Cells
4+
0
4+
0
0

Category: Group l-- Discrepancies that are associated with unexpected or missing/atypically weakened reactions in the reverse typing.

Most probable ABO type: Group A

Possible causes: Immunocompromised, age-related, transplantation, dilution, technical/procedural error

Some strategies for resolution: Repeat testing with careful attention to accuracy or repeat reverse typing with extended incubation at or below room temperature. Weak ABO reactions can often be potentiated using extended incubation at or below room temperature.