The page below is a sample from the LabCE course ABO Typing Discrepancies. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Reagents and Techniques/Testing Methods Used in ABO Discrepancy Resolution

Specific reagents and techniques/methods of testing are used in the investigation and resolution of an ABO discrepancy. Knowing the correct reagents and techniques to employ will result in a more effective and efficient resolution. Some of the reagents and techniques/methods of testing for ABO discrepancy resolution are listed below and will be discussed in more detail on the following pages.
  • Anti-A1 lectin (Dolichos biflorus)
  • Anti-H lectin (Ulex europaeus)
  • A2 reagent red cells
  • O reagent red cells
  • Change incubation temperature
  • Change incubation time
  • Change testing method
When beginning an investigation into an ABO discrepancy, ensure that reagents are not expired and reagents and equipment are working properly. Quality control testing is essential. Additionally, technicians should make sure that the patient sample has been properly collected and labeled according to blood bank protocols.