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The page below is a sample from the LabCE course Minimizing Pre-Analytical Variability During Venipuncture and Urine Sample Collection Processes. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Freeze-Thaw Cycling

Certain components of interest are sensitive to degradation by the process of freezing and subsequent thawing.
One of the more commonly measured enzymes, alanine aminotransferase (ALT), does not withstand the thawing process and freezing may result in falsely low levels and should be avoided.
The immunoglobulins (IgA, IgG, IgM) are also known to decrease upon repeated freeze-thaw cycles, while plasma renin levels are known to increase after a freeze-thaw cycle.