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Conclusion

Red cell antibodies must be properly identified in order to provide compatible blood for transfusion. Many identification techniques are available and policies can vary in different laboratories. It's important to follow your laboratory's Standard Operating Procedures (SOP). The table below summarizes antibody characteristics for most commonly encountered antibodies, including the optimal techniques that were discussed in this course and which antibodies will often exhibit dosage.

AntibodySpecificity

Ig Class

Optimal Technique

Often Exhibits Dosage?

Anti- D

Most IgG; Some IgM

37o C; IAT; Enzymes

No

Anti-C

IgG; IgM

37o C; IAT; Enzymes

Yes

Anti-c

Most IgG; Some IgM

37o C; IAT; Enzymes

Yes

Anti-E

IgG; IgM; Many Naturally Occurring

37o C; IAT; Enzymes

Yes

Anti-e

Most IgG; Some IgM

37o C; IAT; Enzymes

Yes

Anti-Cw

IgG; IgM; Often Naturally Occurring

RT; IAT; Enzymes

No

Anti-K

Most IgG; Few IgM

Usually IAT; Few RT

No

Anti-k

Most IgG; Few IgM

IAT

No

Anti-Kpa

IgG

IAT

No

Anti-Jsa

Most IgG; Some IgM

IAT

No

Anti-Fya

Most IgG; Rare IgM

IAT

Yes

Anti-Fyb

Most IgG; Rare IgM

IAT

Yes

Anti-Jka

IgG; IgM; Many IgG + IgM

IAT; Enzymes: PEG

Agglutination often weak and fragile.

Yes

Anti-Jkb

IgG; IgM; Many IgG + IgM

IAT; Enzymes; PEG

Yes

Anti-Lea

Most IgM; Some IgG

RT; 37°C; IAT; Enzymes

No

Anti-Leb

Most IgM; Some IgG

RT; 37°C; IAT; Enzymes

No

Anti-P1

IgM; Rare IgG

RT or below

No

Anti-M

IgG; IgM

4o C and RT; rare IAT

Yes

Anti-N

IgG; IgM

4o C and RT; rare IAT

Yes

Anti-S

IgG; some IgM

RT and IAT

Yes

Anti-s

IgG; IgM

IAT

Yes

Abbreviations: IAT = indirect antiglobulin test; RT = room temperature