Red cell antibodies must be properly identified in order to provide compatible blood for transfusion. Many identification techniques are available and policies can vary in different laboratories. It's important to follow your laboratory's Standard Operating Procedures (SOP). The table below summarizes antibody characteristics for most commonly encountered antibodies, including the optimal techniques that were discussed in this course and which antibodies will often exhibit dosage.
Anti- D | Most IgG; Some IgM | 37o C; IAT; Enzymes | No
|
Anti-C | IgG; IgM | 37o C; IAT; Enzymes | Yes
|
Anti-c | Most IgG; Some IgM | 37o C; IAT; Enzymes | Yes
|
Anti-E | IgG; IgM; Many Naturally Occurring | 37o C; IAT; Enzymes | Yes
|
Anti-e | Most IgG; Some IgM | 37o C; IAT; Enzymes | Yes
|
Anti-Cw | IgG; IgM; Often Naturally Occurring | RT; IAT; Enzymes | No |
Anti-K | Most IgG; Few IgM | Usually IAT; Few RT | No
|
Anti-k | Most IgG; Few IgM | IAT | No |
Anti-Kpa | IgG | IAT | No |
Anti-Jsa | Most IgG; Some IgM | IAT | No |
Anti-Fya | Most IgG; Rare IgM | IAT | Yes
|
Anti-Fyb | Most IgG; Rare IgM | IAT | Yes
|
Anti-Jka | IgG; IgM; Many IgG + IgM | IAT; Enzymes: PEG Agglutination often weak and fragile.
| Yes
|
Anti-Jkb | IgG; IgM; Many IgG + IgM | IAT; Enzymes; PEG
| Yes
|
Anti-Lea | Most IgM; Some IgG | RT; 37°C; IAT; Enzymes | No
|
Anti-Leb | Most IgM; Some IgG | RT; 37°C; IAT; Enzymes | No
|
Anti-P1 | IgM; Rare IgG | RT or below | No
|
Anti-M | IgG; IgM | 4o C and RT; rare IAT | Yes
|
Anti-N | IgG; IgM | 4o C and RT; rare IAT | Yes
|
Anti-S | IgG; some IgM | RT and IAT | Yes
|
Anti-s | IgG; IgM | IAT | Yes
|
Abbreviations: IAT = indirect antiglobulin test; RT = room temperature
