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The page below is a sample from the LabCE course Immune Hemolytic Anemias. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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WAIHA Investigation: Serological Findings Following Elution

A summary of the possible serological findings of an WAIHA investigation following elution is presented in the table below.

Scenario
DAT Result (IgG and/or Complement)
Serum/Plasma Antibody Screen/Identification
Adsorption
Warm autoantibody reactive in eluate only
Positive Negative
Not indicated
Warm autoantibody reactive in serum/plasma and eluate; no underlying alloantibodies
Positive
Reactive with all panel cells tested
No reactivity after adsorption
Warm autoantibody reactive in serum/plasma and eluate; underlying alloantibodies that must be identified
Positive
Reactive with all panel cells tested
Reactivity after adsorption